|
KMID : 0545120070170030461
|
|
Journal of Microbiology and Biotechnology
2007 Volume.17 No. 3 p.461 ~ p.467
|
|
|
Expression of Enterotoxin Genes in Staphylococcus aureus Isolates Based on mRNA Analysis
|
|
Lee Young-Duck
Moon Bo-Youn
Park Jong-Hyun
Chang Hyo-Ihl
|
|
|
Abstract
|
|
|
|
Staphylococcus aureus strains are important foodborne pathogens that produce various toxins. To evaluate the risk of the enterotoxins, four S. aureus strains from kimbap and two clinical samples were isolated and identified, and their expression of the enterotoxin genes analyzed using a reverse transcription real-time PCR. Various enterotoxin genes were detected, including sea, seg, seh, sei, sen, seo, and sem, where each isolate contained one or two. When the mRNA detection of the enterotoxin genes was analyzed using a reverse transcriptase PCR, various levels of expression were found depending on the species and enterotoxin gene. Therefore, it is reasonable to suggest that the poisoning risk of S. aureus can be effectively evaluated based on the gene expression at the mRNA level.
|
|
|
KEYWORD
|
|
|
S. aureus, food isolates, enterotoxin expression, real-time PCR
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|